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Service Targeting Constructs

  1. Provide us with the gene name (NCBI Acc. No.) and kind of modification.
  2. We develop an in silico strategy and provide you with the electronic data for cross checking.
  3. We order the appropriate BAC clone based on the mouse strains C57/BL6 or 129Sv.
  4. We clone ≤18kb of the modified allele into a high-copy vector backbone, providing large (>5kb) homology arms for an efficient ES cell recombination. Constructs ≤20kb are available as low copy plasmids upon request.
  5. We confirm integrity of the final targeting construct by sequencing.
  6. You receive an E. coli glycerol stock harboring the construct and a detailed report.

Service Strain Optimization

  1. Provide us with a reference sequence file and your E. coli strain*.
  2. We will help to optimize the project design and provide you with electronic data for cross checking.
  3. We confirm clone integrity by sequencing.
  4. You receive an E. coli glycerol stock and a detailed report.

Gene Bridges Contact

+49 6221 13708 11
info@genebridges.com

 

 

 

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