E. coli Strain Modifications
How can Red/ET Recombination help you to optimize E. coli strains?
Escherichia coli is frequently used as model organism and functions as microbial factory in biotechnology. Recombination with Red/ET enables a defined and rapid access to chromosomal modifications:
- Gene disruption, deletion, insertion, modification
- Reporter gene or tag integration
- Promoter fine tuning
Markerless knock-out of E. coli genes
In combination with Flp/Cre technology multiple markerless genome modifications can be achieved.
For more E. coli modification examples please click the thumbnails in the right column.