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706-Cre;tet
Cre Expression Plasmid with tetracycline resistance marker for use in E.coli only
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Description
706-Cre plasmid is designed for use in Cre-mediated genomic manipulations. The plasmid has a pSC101 origin which maintains low copy and replicates at 30°C. The plasmids will not propagate and will get lost when incubated at 37°C. The expression of the Cre-recombinase is driven by the thermosensitive promoter cI578 (λPR promoter). Therefore, the expression of Cre is repressed at 30°C and induced between 37-42°C. The plasmid carries a tetracycline resistance. Note: The sequence of 706-Cre was compiled from information found in the sequence databases, published literature, and other sources, together with partial sequences obtained by Genebridges. This vector has not been completely sequenced. The digestion patterns for BamHI, EcoRI, HindIII, PstI, XbaI and XhoI are indicated.
Contents
- 706-Cre: expression plasmid for Cre recombinase (0.2 ug/ul, 20 ul)
- Manual
