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pCAGGS-FLPe

Enhanced Eukaryotic FLP Expression Plasmid optimized for 37°C (Academia).
The use of the this product is governed by the terms and conditions of the pCAGGS-FLPe Material Transfer Agreement.

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Description

pCAGGS-FLPe-IRESpuro is an improved vector for expression of an enhanced FLP recombinase in mouse ES cells and oocytes. FLPe shows an enhanced thermo stability at 37°C compared to the normal FLP recombinase. After micro-injection of pCAGGS-FLPe into oocytes approximately one-third of heterozygotic mice born show complete FLP recombination. pCAGGS-FLPe-IRESpuro carries a strong, constitutive expression cassette for an in vitro-evolved FLP recombinase under the control of the chicken-s-actin promoter and an hCMV immediate early enhancer. The use of the chimeric CMV enhancer/s-actin promoter leads to a ubiquitous expression profile in eukaryotes. The FLPe recombinase carries a minimal, nuclear localization signal from the SV40 larger T antigen and is operatively linked by an IRES to a puromycin resistance gene. Additional elements of the expression cassette are an intron with splice donor and acceptor sites upstream of the FLPe gene and a bovine growth hormone polyadenylation signal downstream of the puromycin resistance gene. The pCAGGS-FLPe plasmid is appropriate for efficient excision of DNA stretches flanked by FRT sites e.g. for excision of a resistance cassette in a conditional allele in eukaryotic cells. The plasmid carries an ampicillin resistance cassette.

Contents

  • pCAGGS-FLPe: expression plasmid for FLPe recombinase (0.2 ug/ul, 20 ul)
  • Manual



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