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E. coli strain GB08-red
Recombineering-proficient E. coli strain for linear plus circular homologous recombination
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Features
- Recombineering-proficient E. coli strain
- E. coli strain harboring an arabinose inducible γβαA operon (redγ, redβ, redα and recA) at the ybcC locus
Applications
- All types of "linear plus circular" homologous recombination experiments, e.g. plasmid modifications
Description
E. coli strain GB08-red enables recombineering of plasmids and BACs without the necessity to maintain an expression plasmid in the cells since the necessary genes are located on the chromosome.
Contents
- Glycerol stock of recombineering-proficient E. coli strain GB08-red
- Linear control insert (PGK-gb2-neo PCR product)
- Circular control plasmid (high copy plasmid containing 15kb of the mouse Hoxa11 gene)
- Protocol
