Back to list

pCAGGS-Cre

Eukaryotic Cre recombinase expression plasmid.

Manual [PDF]      Contact Support      Order     

Description

Site-specific recombinases (SSRs) like Cre, FLP or Dre are valuable tools in functional genomics and have been applied in various organisms. They mediate recombination between target sites of 32-34 base pairs (bp) in length. The target sites, which are called loxP, FRT or rox sites are 13-14 bp palindromes separated by spacers:

loxP	5-ATAACTTCGTATAATGTATGCTATACGAAGTTAT-3
rox	5-TAACTTTAAATAATGCCAATTATTTAAAGTTA-3
FRT	5-GAAGTTCCTATTCTCTAGAAAGTATAGGAACTTC-3

Recognition sites of the site-specific recombinases Cre (loxP), Dre (rox) and FLP (FRT).

Cre recombinase, which was originally isolated from coliphage P1, mediates recombination between two loxP-sites through the spacer regions.

Our pCAGGS expression vector carries Cre under the control of the chicken-ß-actin promoter and an hCMV immediate early enhancer. The use of the chimeric CMV enhancer/ß-actin promoter leads to a ubiquitous expression profile in eukaryotes. The addition of a Sv40 Large T nuclear localization sequence (nls) further improves the performance in mammalian cells (Schaft J. et al., 2001). The recombinases are linked to a puromycin resistance gene by an internal ribosomal entry site (IRES).

The pCAGGS-Cre Plasmid allows efficient excision of DNA stretches flanked by loxP sites. The plasmid carries a puromycin resistance gene for selection in eukaryotic cells and an ampicillin resistance cassette for selection in E. coli.

Map of pCAGGS-Cre

Contents

• Recombinase expression plasmid pCAGGS-Cre (0.2 µg/µl, 20 µl)
• Manual