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707-FLPe, tet

Flp Expression Plasmid with tetracycline resistance marker for use in E.coli only

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Description

FLPe expression plasmids 707-710 (see below) enable FLP-mediated site-directed recombination in E. coli, and are propagated at 30°C in low copy number. The flpe gene is driven by the λR promoter and is under control of the heat labile cI857 repressor. A temperature upshift from 30°C to 37°C results in transient FLPe recombinase expression. Since the expression plasmids are no longer replicated at 37°C due to their pSC101 origin of replication they finally get lost. FLPe has an improved thermostability and shows enhanced recombinase activity at 37 – 40°C compared to the wild type FLP protein (Buchholz F., Angrand P.-O. and Stewart A.F., 1998).

Available FLPe Expression Plasmids

Name Cat. No. Resistance marker Recommended drug conc.
707-FLPe A104 Tetracycline 3 µg/ml
708-FLPe A105 Chloramphenicol 15 µg/ml
709-FLPe A106 Ampicillin 50 µg/ml
710-FLPe A107 Kanamycin 15 µg/ml

Contents

  • Expression plasmid encoding for FLPe recombinase (0.2 µg/µl, 20 µl)
  • Manual



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