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709-FLPe, ap
Flp Expression Plasmid with ampicillin resistance marker for use in E.coli only
Manual [PDF] Contact Support Order
Description
FLPe expression plasmids 707-710 (see below) enable FLP-mediated site-directed recombination in E. coli, and are propagated at 30°C in low copy number. The flpe gene is driven by the λR promoter and is under control of the heat labile cI857 repressor. A temperature upshift from 30°C to 37°C results in transient FLPe recombinase expression. Since the expression plasmids are no longer replicated at 37°C due to their pSC101 origin of replication they finally get lost. FLPe has an improved thermostability and shows enhanced recombinase activity at 37 – 40°C compared to the wild type FLP protein (Buchholz F., Angrand P.-O. and Stewart A.F., 1998).
Available FLPe Expression Plasmids
| Name | Cat. No. | Resistance marker | Recommended drug conc. |
|---|---|---|---|
| 707-FLPe | A104 | Tetracycline | 3 µg/ml |
| 708-FLPe | A105 | Chloramphenicol | 15 µg/ml |
| 709-FLPe | A106 | Ampicillin | 50 µg/ml |
| 710-FLPe | A107 | Kanamycin | 15 µg/ml |
Contents
- Expression plasmid encoding for FLPe recombinase (0.2 µg/µl, 20 µl)
- Manual
